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However, the first ‘non-academic’ microfluidic application of hydrodynamic focusing was in the area of flow cytometry, a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. Hydrodynamic focusing, where the core flow of investigated sample is sheathed by an inert fluid, is used in flow cytometry as a way to deliver the sample of suspended cells to the analyzed region in an appropriate form. , 2005; Givan, 2011; Shapiro, 2003). Focused stream residing in a channel centre gives a new possibility – to control the focused sheet position by changing the ratio of side streams, which was quickly utilized in the area of µ-TAS (micro-total-analysis systems).

The future development showed more flexibility and advantages of such continuous flow reactor design. , 2004 investigated the possibilities of hydrodynamic focusing application in generation of liposome vesicles. Liposomes, being a class of nanoparticles encapsulating the aqueous volume by phospholipid bilayer, play a crucial role in biotechnology and life sciences delivering drugs or genetic material into a cell. Jahn et al. (2004) took advantage of the laminar character of microfluidic flow, because the lack of temperature, shear stress and composition fluctuations in the reaction environment causes that the product can be characterized by high monodispersity compared to bulk 32 Advances in Microfluidics produced liposomes – important progress bearing in mind that the vesicle size is one of the basic liposome characteristics determining the quantity of encapsulated material.

Trans. R. Soc. Lond. A 362: 923–935. , Teitell, M. A. -Y. (2011). High-speed droplet generation on demand driven by pulse laser-induced cavitation, Lab Chip 11: 1010–1012. , Lereu, A. , Farahi, R. , Ferrell, T. L. & Thundat, T. (2006). Nonradiative surface plasmon assisted microscale Marangoni forces, Phys. Rev. E 73: 066311. 066311 Piazza, R. & Parola, A. (2008). Thermophoresis in colloidal suspensions, J. : Condens. Matter 20: 153102. , Wunenburger, R. -P. (2008). Laser switching and sorting for high speed digital microfluidics, Appl.

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